Review Artikel: Teknik -Teknik Analisis Profil Mikrobiota Penyebab Karies
DOI:
https://doi.org/10.35965/eco.v22i3.2106Keywords:
Mikrobioma Rongga Mulut, Karies, Teknik AnalisaAbstract
Karies gigi merupakan penyakit infeksi kronis yang umum terjadi akibat bakteri kariogenik yang melekat pada gigi. Mikroorganisme kariogenik berperan penting dalam perkembangan karies. Streptococcus mutan merupakan mikroorganisme pathogen penting dalam perkembangan lesi karies. Perkembangan terbaru dalam teknologi sekuensing DNA belakangan ini telah memungkinkan untuk memetakan profil komposisi mikrobioma rongga mulut dan memberikan harapan dalam menyelesaikan penyakit menular ini. Pada penelitian ini, kami mengumpulkan data penelitian mengenai teknik analisis profil mikrobioma rongga mulut melalui mesin pencarian online Google Scholar dan Pubmed. Dan didapatkan 15 jurnal yang membahas mengenai teknik identifikasi mikrobioma rongga mulut. Teknik analisis mikrobioma rongga mulut dapat diketahui dengan jelas beserta kelebihan dan kekurangan untuk merancang penelitian lebih lanjut tentang mikrobioma penyebab terjadinya karies.
Dental caries is a common chronic infectious resulting from tooth adherent cariogenic bacteria. The cariogenic microorganisms play an important part in caries development. Streptococcus mutan is an important pathogenic organism in the development of caries lesion. The development of dental caries is highly associated with the microbiota in the oral cavity. Recent developments in DNA sequencing technology have made it possible to map the compositional profile of the oral microbiome and provide hope in solving the problem on this infectious disease. The development of dental caries is closely related to the microbiota in the oral cavity. Recent developments in DNA sequencing technology have made it possible to map the compositional profile of the oral microbiome and provide hope in solving this infectious disease problem. Methode: In this study, we collected research data regarding the technique of analyzing oral microbiome profiles through the online search engines Google Scholar and Pubmed. And obtained 15 journal that discuss the identification technique of the oral microbiome Result: The oral microbiome analysis technique can be identified clearly along with its advantages and disadvantages for design further research on the microbiome that causes caries.
Downloads
References
Amalia R, Chairunisa F, Alfian MF, Supartinah A. (2019). Indonesia: Epidemiological Profiles of Early Childhood Caries. Front Public Health. 6,7, 210. doi: 10.3389/fpubh.2019.00210. PMID: 31448251; PMCID: PMC6691044.
Balolong, M. P., & Mendoza, M. A. F. (2020). Understanding Oral Diseases: Exploring Opportunities from Filipino Oral Microbiome Research. In (Ed.), Dental Caries. IntechOpen.
Berkowitz RJ, Amante A, Kopycka-Kedzierawski DT, Billings RJ, Feng C. (2011). Dental caries recurrence following clinical treatment for severe early childhood caries. Pediatr Dent, 33(7):510-4. PMID: 22353412.
Cullen CM, Aneja KK, Beyhan S, Cho CE, Woloszynek S, Convertino M, McCoy SJ, Zhang Y, Anderson MZ, Alvarez-Ponce D, Smirnova E, Karstens L, Dorrestein PC, Li H, Sen Gupta A, Cheung K, Powers JG, Zhao Z, Rosen GL. (2020). Emerging Priorities for Microbiome Research. Front Microbiol, 19;11:136. doi: 10.3389/fmicb.2020.00136. PMID: 32140140; PMCID: PMC7042322.
Do T, Dame-Teixeira N, Deng D. (2021). Editorial: Applications of Next Generation Sequencing (NGS) Technologies to Decipher the Oral Microbiome in Systemic Health and Disease. Front Cell Infect Microbiol, 22;11:801122. doi: 10.3389/fcimb.2021.801122. PMID: 35004359; PMCID: PMC8727447.
J. Choubey, J.K. Choudhari, B.P. Sahariah, M.K. Verma, A. Banerjee. (2021) Chapter 25 - Molecular Tools: Advance Approaches to Analyze Diversity of Microbial Community. (pp:507-520). Elsevier.
Jaksik R, Iwanaszko M, Rzeszowska Wolny J, Kimmel M. (2015). Microarray experiments and factors which affect their reliability. Biol Direct, doi: 10.1186/s13062-015-0077-2. PMID: 26335588; PMCID: PMC4559324.
Jian C, Luukkonen P, Yki-Järvinen H, Salonen A, Korpela K. (2020). Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling. PLoS One, doi: 10.1371/journal.pone.0227285. PMID: 31940382; PMCID: PMC6961887.
Ibrahim S, Nishimura M, Matsumura S, Rodis O.M.M, Nishida A, Kariya N, Yamanaka K, Shimono T. (2009). Microbial screening of cariostat-inoculated plaque samples from low and high caries risk (vol. 19, pp: 181-186). Dental Journal.
Krishnan K, Chen T, Paster BJ. (2017). A practical guide to the oral microbiome and its relation to health and disease. Oral Dis, doi: 10.1111/odi.12509
Martín R, Miquel S, Langella P, Bermúdez-Humarán LG. (2014). The role of metagenomics in understanding the human microbiome in health and disease. Virulence, doi: 10.4161/viru.27864.
Muhamad Rizal NS, Neoh HM, Ramli R, A/L K Periyasamy PR, Hanafiah A, Abdul Samat MN, Tan TL, Wong KK, Nathan S, Chieng S, Saw SH, Khor BY. (2020). Advantages and Limitations of 16S rRNA Next-Generation Sequencing for Pathogen Identification in the Diagnostic Microbiology Laboratory: Perspectives from a Middle-Income Country. Diagnostics (Basel), doi: 10.3390/diagnostics10100816. PMID: 33066371; PMCID: PMC7602188.
Rosier BT, De Jager M, Zaura E, Krom BP. (2014). Historical and contemporary hypotheses on the development of oral diseases: are we there yet?. Front Cell Infect Microbiol, doi: 10.3390/diagnostics10100816. PMID: 33066371; PMCID: PMC7602188.
Yang, F., Zeng, X., Ning, K. et al (2012). Saliva microbiomes distinguish caries-active from healthy human populations. ISME J 6, 1–10
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Sukaeni Ibrahim, Marhaen Hardjo
This work is licensed under a Creative Commons Attribution 4.0 International License.