ANALISIS EKSTRAK ABALON TROPIS HALIOTIS ASININA TERHADAP GAMBARAN REGENERASI LUKA SIRIP KAUDAL IKAN NILA OREOCHROMIS SP

Nona Mu'minun; Sutia Budi; Erni Indrawati

doi:10.35965/jae.v6i2.3135

Authors

Nona Mu'minun Fakultas Keperawatan, Megarezky University Makassar
Sutia Budi Program Studi Budidaya Perairan, Program Pascasarjana, Universitas Bosowa
Erni Indrawati Program Studi Budidaya Perairan, Program Pascasarjana, Universitas Bosowa

DOI:

https://doi.org/10.35965/jae.v6i2.3135

Keywords:

Regenerasi, Sirip Ikan Nila, Visceral, Mucus

Abstract

Penelitian ini bertujuan untuk menganalisis efektivitas ekstrak visceral abalon tropis haliotis asinine dalam percepatan regenerasi luka sirip kaudal ikan nila (Oreochromis) dan menganalisis efektivitas simplisia mucus abalon tropis haliotis asinine dalam percepatan regenerasi luka sirip kaudal ikan nila (Oreochromis). Penelitian ini dilaksananakan di bulan Juli tahun 2023 bertempat di bertempat di Lembaga Pengkajian dan Penerapan Teknologi Sumberdaya Perikanan dan Kelautan (LP2T-SPK) Konawe Sulawesi Tenggara. Rancangan penelitian ini adalah eksperimen pre post test only control group design dengan uji analisis data menggunakan uji nonparametrik yaitu Uji Kruskal Wallis. Hasil analisis penelitian menunjukan bahwa ekstrak visceral abalon tropis Haliotis asinina terbukti paling efektif dalam mempercepat regenerasi histologi luka sirip kaudal pada ikan nila (Nila oreochromis) dibandingkan dengan kelompok perlakuan mucus dan kelompok kontrol dengan tingkat kemaknaan (U = 0,000 p = 0,000).

This study aimed to analyze the effectiveness of the visceral extract of tropical abalone Haliotis asinina in accelerating the regeneration of caudal fin wound of tilapia (Oreochromis). This research was carried out in July 2023 at the Institute for the Assessment and Application of Fisheries and Marine Resources Technology (LP2T-SPK) Konawe, Southeast Sulawesi. The design of this study was an experimental pre post test only control group design with data analysis using a nonparametric test, namely the Kruskal Wallis Test. The resulth of the study showed that the visceral extract of tropical abalone Haliotis asinina proved to be the most effective in accelerating the histological regeneration of caudal fin wounds of tilapia (Nila oreochromis) compared to the mucus treatment group and the control group with a significance level (U = 0.000 p = 0.000).

Downloads

Download data is not yet available.

References

Azevedo, A.S., Grotek, B., Jacinto, A., Weidinger, G., Saúde, L., 2011. The Regenerative Capacity Of The Zebrafish Caudal Fin Is Not Affected By Repeated Amputations. Plos One 6, E22820. Https://Doi.Org/10.1371/Journal.Pone.0022820

Afizia, W.M & Rosida (2012) Potensi Ekstrak Jambu Biji Sebagai Hydrophyla Pada Ikan Gurami (Osphorenemus Gouramy Pacopede). Jurnal Akuatika, 3(1), 19-27.

Campbell, N.A., Reece, J.B., Urry, L.A., Cain, M.L., Wasserman, S.A., Minorsky, P.V., dan Jackson, R. B. 2010. Biologi Jilid 3. Edisi 8. (Terjemahan oleh D T Wulandari). Erlangga. Jakarta. 486 hlm.

Catherine Pfefferli, Anna Jazwinska., (2015) The art of fin regeneration in zebrafish., PMID: 27499869.,PMCID: PMC4895310. DOI: 10.1002/reg2.33.

Cardeira, J. Et Al. Quantitative Assessment Of The Regenerative And Mineralogenic Performances Of The Zebrafish Caudal Fin. Sci. Rep. 6, 39191; DOI: 10.1038/Srep39191 (2016).

Chablais, F., Jazwinska, A, 2010. IGF Signaling Between Blastema and Wound Epidermis Is Required For Fin Regeneration. Development. 137, 871–879.

Chaudhari M, Mengi S. Evaluation of phytoconstituents of Terminalia arjuna for wound healing activity in rats. Phytother Res. 2006;20(9):799-805.

Effendy, I.J., J. Hutabarat., A Ambariyanto and F. Basuki. (2018). Protein content and free amino acid composition of abalon (Haliotis asinina) broodstock fed by different fresh macroalgae and formulated diet. AACL Bioflux, 2018, 11(3).

Effendy. I.J. (2018). Kinerja Reproduksi, Kualitas Telur Dan Kualitas Larva Dari Induk Abalon (Haliotis Asinina Linnaeus, 1758) Yang Diberi Pakan Alami Dan Pakan Formulasi. Disertasi. Manajemen Sumber Daya Pantai. Fakultas Perikanan dan Ilmu Kelautan. Universitas Diponegoro. Semarang. Indonesia.

Geiger, D. L, 2005. Molecular Phylogeni And The Geograpic Orogin Of Haliotidae Traced By Haemocyanin Sequences, Journal Of Molluscan Studies Advance. Santa Barbara Museum Of Natural History. Pp. 1-6.

Goldshmith, Y., Sztal, T.E., Jusuf, P.R., Hall, T.E., Nguyen-Chi, M., Currie, P.D., 2012. Fgf-Dependent Glial Cell Bridger Facilitate Spinal Cord Regeneration In Zebrafish. The Journal Of Neuroscience. 32 (22): 7477-92. Doi:10.1523/JNEUROSCI.0758-12.2012.PMID22649227

Hale, A.J., Kiasi, A Sikkens, J.,den Hertog, J (2017). Ipaired Cudal Fin-Fold Regeneration In Zebrafish Deficient For The Tumor Suppressor Pten., Journal ZDB-PUB-180105-2. PMID: 29299324.

Hadijah., 2017. Mengenal Abalon Tropis Biologi Dan Ekologi: Cetakan I Makassar, CV Sah Media; ISBN 978-602-6928-21-4.

Hann, K.O. 1992. Review of endocrine regulation of reproduction in abalon spp.in: abalon of the word: biology fisheries, and culture (SAshepperd, M.J TEGNER and S.A GUZMAN del PROO eds.). blackwells, oxford: 49-58

Harish BG, Krishna V, Kumar HS, Ahamed KB, Sharath R, Swamy KH. Wound healing activity and docking of glycogen-synthase-kinase- 3-b-protein with isolated triterpenoid lupeol in rats. Phytomedicine. 2008; 15:763-7.

Ho-Seok Rho,Et Al (2015) Anti-Inflammatory Effect Of By-Products From Haliotis Discus Hannai In Raw 264.7 Cells. Hindawi Publishing Corporation Journal Of Chemistry. Volume 2015, Article Id 526439, 7 Pages. Http://Dx.Doi.Org/10.1155/2015/526439.

Indraswari, A., 2008, Optimasi Pembuatan Ekstrak daun Dewandaru (Eugenia uniflora L) mengggunakan Metode Maserasi dengan Parameter Kadar Total Senyawa Fenolik dan Flavonoid, Skripsi, Universitas Muhamadiyah Surakarta, Surakarta.

Iza, N. (2010). Ikan Gatul (Poecilia Sp.) Sebagai Kandidat Hewan Model: Proses Regenarasi Sirip Kaudal (Doctoral Dissertation, Universitas Negeri Malang).

Kawakami, A. (2010), Stem cell system in tissue regeneration in fish. Development, Growth & Differentiation, 52: 77-87. https://doi.org/10.1111/j.1440-169X.2009.01138.xKawakami, A. (2010), Stem cell system in tissue regeneration in fish. Development, Growth & Differentiation, 52: 77-87. https://doi.org/10.1111/j.1440-169X.2009.01138.x

Khairuman, S., And Dr. Khairul Amri, Spi, Msi, Budidaya Ikan Nila, Depok: PT.Agro Media Pustaka, 2013.

Kimball, J. W. 1993. Biologi Umum. Erlangga. Jakarta.

Kordi, K.M.G.H. 2010. Panduan Lengkap Memelihara Ikan Air Tawar Di Kolam Terpal. Yogyakarta: Andi Offset.

Kurahasi T, Fujii J. Roles of antioxidative enzymes in wound healing. J Dev Biol. 2015;3:57-70.

Lush, M.E., Piotrowski, T., 2014. "Sensory Hair Cell Regeneration In The Zebrafish Lateralline". Developmental Dynamics. 243 (10): 1187-202. Doi:10.1002/Dvdy.24167. PMC 4177345. PMID 25045019.

Marisa Jusie Octaviany. 2007. Catatan Tentang Aspek Biologi Dan Perikanan Abalone. Volume XXXII: 39- 47 2. Cabi. 2019. Https://Www.Cabi.Org/Isc/Datasheet/81161.

Muralidhar A, Babu KS, Sankar TR, Reddanna P, Latha J. Wound healing activity of flavonoid fraction isolated from the stem bark of Butea monosperma (Lam) in albino wistar rats. Eur J Exp Biol. 2013;3(6):1-6

Octaviany, M. J. 2007. Beberapa Catatan Tentang Aspek Biologi Dan Perikanan Abalon. Oseana, 32 (4): 39-47.

Pathan, M. A., Chaudhari, A., & Krishna, G. (2019). Inbred Zebrafish Lines: A Genetic Repository For Zebrafish Researchers. Indian J. Genet, 79(1 Suppl 150), 159.

Prayitno, S.B (2014) Pathogenisitas Aeromonas Hydrophylayang Diisolasi Dari Lele Dumbo (Glarias Gariepinus) Yang Berasal Dari Boyolal. Jurnal Of Aquaculture management And Technology 3(2), 11-7.

Rusyana, A. (2011).Zoologi Invertebrata. Bandung: Alfabet.

Realita, Filza Yulina Ade, Dahlia., https://media.neliti.com/media/publications/109870-ID-jenis-jenis-ikan-segar-yang-diperdagangk.pdf

Rusdi, I., A. Hanafi., B. Susanto., dan M. Marzuqi. 2010. Peningkatan Sintasan Benih Abalon Haliotis Squamata 01 Hatchery Melalui Optimalisasi Pakan dan Lingkungan. BBRPBL. Bali. hal. 7, 8, 25, 26.

Santoso, S. (2010). Statistik parametrik. Elex Media Komputindo.

Saparinto, C. 2011. Usaha Ikan Konsumsi Di Lahan 100 M2 . Jakarta. Penebar Swadaya.

Sari, Nila Kartika, Listyorini, Dwi., Gofur, Abdul. (2016); Proses Regenerasi Sirip Ekor Pada Ikan Zebra., EDUBIOTIK ISSN: 2528 ± 679X; Vol. 1 No. 1: Hal. 25-29 September 2016.

Sari, F.S Dkk. (2020), Antioxidant Activity And Bioactive Compound Of Methanol Extract Of Tropical Abalone, Haliotis Asinine. Indonesian Journal Of Fisheries Science And Technology. Vol. 16 No. 2: 104-108, Agustus 2020

Sari D.S Pangastuti, A. & Herawati.E.(2013) Infection Prevention Of Journal Konversi, 5(1),17-23.

Suleria, H.A.R., P.P. Masci., G.C. Gobe., S.A.Osborne. 2015. Therapeutic Potential Of Abalone And Status Of Bioactive Molecules: A Comprehensive Review. J. Critical Reviewsin Food Science And Nutrition, 57(8): 1742-1748.

Susanto, H. 2009. Budidaya Ikan Di Pekarangan (Revisi). Jakarta. Penebar Swadaya.

Suryani. 2006. Budi Daya Ikan Air Tawar. Yogyakarta. Pt Citra Aji Parama.

Sehring I, Weidinger G., (2022)., Zebrafish Fin: Complex Molecular Interactions and Cellular Mechanisms Guiding Regeneration., Cold Spring Harb Perspect Biol. 2022 Jul 1;14(7):a040758. doi: 10.1101/cshperspect.a040758.PMID: 34649924 Review.

Sfakianakis, D. G., Leris, I., Laggis, A., & Kentouri, M. (2011). The Effect Of Rearing Temperature On Body Shape And Meristic Characters In Zebrafish (Danio Rerio) Juveniles. Environmental Biology Of Fishes, 92(2), 197.

Soni H, Singhai AK. A recent update of botanicals for wound healing activity. Int Res J Pharm. 2012;3:1-6.

Stewart, S., Tsun, Z.Y., Izpisua Belmonte, J.C., 2009. " A Histone Demethylase Is Necessary For Regeneration In Zebrafish". Proceedings Of The National Academy Of Sciences Of The United States Of America. 106(47): 19889-94. BIBCODE: 2009 PNAS.. 10619889s. Doi:10,1073/Pnas.0904132106. Jstor25593294. PMC 2785262. PMID 19897725

Tiwari, P., Kumar, B., Kaur, M., Kaur G. & Kaur H., 2011, Phytochemical Screening And Extraction: A Review, International Pharmaceutica Sciencia, 1 (1), 98-106.

TOM, P.D. 2007. Abalone. Seafood Network Information Center. http:// seafood.ucdavis.edu/. Tanggal akses 11 Desember 2022.

Tu, S., & Johnson, S. L. (2011). Fate Restriction In The Growing And Regenerating Zebrafish Fin. Developmental Cell, 20(5), 725–732. Doi:10.1016/J.Devcel.2011.04.013

Utami, N. (2018). Zebrafish (Danio rerio) Sebagai Hewan Model Diabetes Mellitus. Biotrends, 9(1), 15-19.

Wardhana, A., A. Husein., dan J. Manurung. 2005. Efektifitas Ekstrak Biji Srikaya (Annona squamosa L) dengan Pelarut Air, Metanol dan Heksan terhadap Mortalitas Larva Caplak Boophilus microplus secara In Vitro. Bogor: Balai Penelitian Veteriner.